• Users Online: 508
  • Print this page
  • Email this page

 Table of Contents  
Year : 2018  |  Volume : 30  |  Issue : 1  |  Page : 5-9

Delayed formation of hematomas with ethanol preconditioning in experimental intracerebral hemorrhage rats

1 Department of Physical Medicine and Rehabilitation, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
2 Institute of Medical Sciences, Tzu Chi University; Department of Radiology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
3 Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan
4 Master Program and PhD Program in Pharmacology and Toxicology, Tzu Chi University, Hualien, Taiwan
5 Institute of Medical Sciences, Tzu Chi University; Department of Medical Research, Buddhist Tzu Chi General Hospital, Hualien, Taiwan

Date of Submission13-Jul-2017
Date of Decision11-Aug-2017
Date of Acceptance29-Sep-2017
Date of Web Publication27-Feb-2018

Correspondence Address:
Prof. Cheng-Yoong Pang
Department of Medical Research, Buddhist Tzu Chi General Hospital, 707, Section 3, Chung-Yang Road, Hualien
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/tcmj.tcmj_184_17

Rights and Permissions

Objective: Spontaneous intracerebral hemorrhage (ICH) accounts for 10%–15% of all strokes and causes high mortality and morbidity. In the previous study, we demonstrated that ethanol could aggravate the severity of brain injury after ICH by increasing neuroinflammation and oxidative stress. In this study, we further investigate the acute effects of ethanol on brain injury within 24 h after ICH. Materials and Methods: Totally, 66 male Sprague-Dawley rats were assigned randomly into two groups: saline pretreatment before ICH (saline + ICH), and ethanol pretreatment before ICH (ethanol + ICH). Normal saline (10 mL/kg) or ethanol (3 g/kg, in 10 mL/kg normal saline) was administered intraperitoneally 1 h before induction of experimental ICH. Bacterial collagenase VII-S (0.23 U in 1.0 μL sterile saline) was injected into the right striatum to induce ICH in the rats. We evaluated the hematoma expansion, hemodynamic parameters (heart rate and blood pressure), activated partial thromboplastin time (aPTT), prothrombin time (PT), and striatal matrix metallopeptidase 9 (MMP-9) expressions at 3, 6, 9, and 24 h after ICH. Results: The ethanol + ICH group exhibited decreased hematoma at 3 h after ICH; nevertheless, there was a larger hematoma compared with the saline + ICH group at 9 and 24 h after ICH. The ethanol + ICH group had lower blood pressure at 3, 6, and 9 h post-ICH, but both groups maintained similar heart rates after ICH. There was no significant difference in the aPTT and PT between the two groups. Incremental ethanol concentrations had no influence on collagenase VII-S activity at 120 min in vitro. MMP-9 expression was upregulated in the right striata of the ethanol + ICH group, especially at 3 and 9 h after ICH. Conclusion: Ethanol delayed hematoma formation in the first 3 h due to a hypotensive effect; however, the accelerated growth of hematomas after 9 h may be a sequela of ethanol-induced MMP-9 activation.

Keywords: Ethanol, Intracerebral hemorrhage, Matrix metallopeptidase-9

How to cite this article:
Cheng HY, Huang LC, Peng HF, Kuo JS, Liew HK, Pang CY. Delayed formation of hematomas with ethanol preconditioning in experimental intracerebral hemorrhage rats. Tzu Chi Med J 2018;30:5-9

How to cite this URL:
Cheng HY, Huang LC, Peng HF, Kuo JS, Liew HK, Pang CY. Delayed formation of hematomas with ethanol preconditioning in experimental intracerebral hemorrhage rats. Tzu Chi Med J [serial online] 2018 [cited 2022 Aug 14];30:5-9. Available from: https://www.tcmjmed.com/text.asp?2018/30/1/5/226241

  Introduction Top

Spontaneous intracerebral hemorrhage (ICH) presenting as bleeding in the brain parenchyma accounts for approximately 10%–15% of all strokes, with an incidence of 4.3 per 10,000 person-years[1]. The high 30-day fatality rate approaches 40% after ICH[2]. The risk factors for ICH include hypertension, alcohol use, current cigarette smoking, and oral anticoagulant and antiplatelet usage[3]. Taylor and Combs-Orme reported binge drinking may enhance all types of strokes among young adults[4]. There is much evidence disclosing how binge drinking can aggravate brain injury[5],[6].

In the previous study, we found prior ethanol treatment could aggravate the mortality and severity of ICH-induced brain injury by inducing oxidative stress and neuroinflammation in experimental rat models[7]. However, the acute effects of ethanol on stroke patients are controversial. Epidemiologic studies suggest that light to moderate ethanol consumption reduces the risk of adverse cerebrovascular events and overall mortality compared with those in abstainers while heavy drinkers (3–4 or more drinks per day) demonstrate increased risks[8],[9]. Wang et al. even proposed that ethanol preconditioning can ameliorate ischemia/reperfusion-induced brain damage by a mechanism that involves mild reactive oxygen species production through nicotinamide adenine dinucleotide phosphate oxidase[10]. Thus, the acute effects of ethanol on ICH-induced brain injury are still unclear.

In ICH animal models, elevated matrix metallopeptidase-9 (MMP-9) contributes to blood–brain barrier (BBB) disruption, perihematomal edema, and neuronal cell death[11]. Li et al., found increased MMP-9 levels on admission were associated with poor clinical outcomes at 90 days in human subjects[12]. MMP-9 could be taken as a molecular marker for the prognosis of the severity and secondary injury in ICH. Chronic ethanol exposure increased cerebral MMP-9 activity and resulted in degradation of tight junctions and extracellular matrix in postmortem human brains[13]. Treating brain microvascular endothelial cells with ethanol also promoted MMP-9 activity at 2–48 h in vitro[14].

To investigate the acute effect of ethanol on ICH, we injected ethanol intraperitoneally (IP) before induction of ICH. The hemodynamic parameters and coagulative function were monitored in free-moving and awake ICH rats. The hematoma volume was evaluated by serial brain slices.

  Materials and Methods Top


All experimental protocols were approved by the Animal Care and Use Committee of Tzu Chi University, Hualien, Taiwan (Approval no. 101-34), in accordance with the guidelines of the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Animals were housed under a 12-h light/dark cycle with free access to food and water. All efforts were made to minimize suffering and the number of animals used.


Totally, 66 male Sprague-Dawley rats were used for our study. Normal saline (10 mL/kg) or ethanol (3 g/kg, in 10 mL/kg normal saline) was administered IP in assigned groups before ICH. A total of 48 rats were distributed to saline pretreatment before ICH (saline + ICH, n = 6), or ethanol pretreatment before ICH (ethanol + ICH, n = 6) for the evaluation of hematoma expansion and western blotting at four separate time points after ICH. Another six rats were sacrificed for brain tissues analysis before experimental ICH as the normal controls. We used another 12 rats for the investigation of hemodynamics and coagulative parameters in the saline + ICH (n = 6) and ethanol + ICH (n = 6) groups for 24-h consecutive monitoring after ICH.

Intracerebral hemorrhage induction

Male Sprague-Dawley rats (300–350 g) were anesthetized with pentobarbital 50 mg/kg IP. Bacterial collagenase VII-S (0.23 U in 1.0 μL sterile saline) was infused through a 2 mm diameter burr hole into the right striatum (0.0 mm posterior, 3.0 mm right, 5.0 mm ventral to the bregma at the skull surface) of the rat over a period of 10 min[15]. The syringe needle was kept in place for another 10 min to prevent backflow. The burr hole was sealed with bone wax, and the rats were allowed to recover in separate cages equipped with a heating pad (CMA-150, CMA Microdialysis, Stockholm, Sweden) kept at 37°C.

Evaluation of hematoma expansion

Morphometric measurement of hematomas was conducted 3, 6, 9, and 24 h after ICH[7]. Briefly, rats were decapitated under deep anesthesia and the brains were rapidly removed. The brains were sliced coronally through the needle entry plane, and then serially sliced into 2-mm thickness. Images were taken by a digital camera. Digital photographs of serial slices were quantified with Image J (NIH, Bethesda, MD, USA). The sliced tissues were also subjected to western blot analysis as indicated below.

Evaluation of blood pressure, platelet count, activated partial thromboplastin time, and prothrombin time

Twelve rats were randomly assigned into the saline + ICH (n = 6) and ethanol + ICH (n = 6) groups for evaluation of mean arterial blood pressure (MABP), heart rate (HR), platelet count, activated partial thromboplastin time (aPTT), and prothrombin time (PT). Under isofluorane anesthesia (initial: 5%, maintain: 2%), the femoral arteries of all rats were cannulated with a PE-50 polyethylene tube for monitoring of arterial blood pressure and heart rate. Femoral veins were cannulated for blood withdrawal for platelet counts, and aPTT and PT assays. After the operations, isofluorane was withdrawn to let all rats recover from anesthesia. The hemodynamic signals were transduced to an amplifier (MP35, BIOPAC System, Inc., Goleta, CA, USA) and collected 10 min before (baseline), and 3, 6, 9, and 24 h, after ICH in these conscious rats.

Collagenase assay

An EnzChek® Gelatinase/Collagenase assay kit was used to measure the effect of ethanol on collagenase activity according to the manufacturer's instruction (E-12055, Molecular Probe, Eugene, OR, USA). Briefly, 0%, 2.5%, 5%, 10%, 20%, 30, and 40% (w/v) of ethanol was mixed with 0.2 U/mL collagenase and incubated for 2 h in DQ collagen solution (100 μg/mL). Fluorescence intensity was measured at 0, 15, 30, 60, 90, and 120 min using a microplate reader set for excitation at 495 nm and emission detection at 515 nm.

Western blotting

Ipsilateral and contralateral striata were dissected from the slices for western blot analysis at 0, 3, 6, 9, and 24 h after ICH insult. Protein concentrations were determined using a Bio-Rad protein assay kit (Bio-Rad Laboratories, Hercules, CA, USA). A total of 50 μg of total protein from each sample was separated by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to an Immobilon®-P polyvinylidene difluoride membrane (Millipore, Bedford, MA, USA). The membranes were then blocked with 5% nonfat milk in 0.05% Tween-Tris-buffered saline. The membranes were probed with various primary antibodies and subsequently with appropriate secondary antibodies. The primary antibodies were anti-MMP 9 antibody (Abcam, Cambridge, MA, USA) and anti-β actin (Becton Dickinson, Franklin Lakes, NJ, USA). The antigen-antibody complexes were visualized with an electrochemiluminescence system (Amersham Bioscience, Buckinghamshire, UK) and exposed to Kodak X-OMAT film (GE Healthcare Limited, Buckinghamshire, UK). The intensity of each band was quantified with a GS-800 calibrated densitometer (Bio-Rad) and calculated as the (optical density)/(fix area of band).

Statistical analysis

Data are presented as mean ± standard deviation. Statistical analysis was performed using independent Student's t-test for hematoma volume and MMP-9 expression, and two-way analysis of variance for hemodynamic and coagulative parameters with Prism Graph 5.0 (GraphPad Software Inc., La Jolla, CA, USA). In all instances, n refers to the number of animals in a particular group. P < 0.05 is considered statistically significant.

  Results Top

During the hyperacute phase, i.e., 3 h post-ICH insult, the ethanol + ICH rats demonstrated a lower hematoma volume (28.6 ± 4.3 mm3) than the saline + ICH rats (43.8 ± 6.9 mm3). The hematoma volume of the ethanol + ICH group increased significantly at 9 h (67.9 ± 16.2 mm3) and 24 h (74.4 ± 3.9 mm3) while the hematoma volumes of the saline + ICH group were 48.6 ± 9.1 mm3 at 9 h and 50.6 ± 7.2 mm3 at 24 h [Figure 1].
Figure 1: Morphometric measurements of intrastriatal hematoma volume. (a) Representative images are shown for saline- and ethanol-pretreated animals at 3, 6, 9, and 24 h after ICH. (b) Quantitative analysis of hematomas at 3, 6, 9, and 24 h after ICH. Data are presented as mean ± SD. *P < 0.05 and **P < 0.01 versus saline + ICH (n = 6 for each group). ICH: Intracerebral hemorrhage, SD: Standard deviation

Click here to view

No significant differences in the baseline MABP, HR, platelet count, PT, and aPTT were found in either group [Table 1]. However, ethanol did cause a significant decrease in the MABP (20–30 mmHg) almost immediately after injection. This decrease was sustained up to 9 h and returned to baseline at 24 h [Table 1]. The platelet count, PT, and aPTT demonstrated no differences between the ethanol + ICH and saline + ICH groups at any time point.
Table 1: Hemodynamic parameters, platelet counts, prothrombin time, and activated partial thromboplastin time of rats with intracerebral hemorrhage

Click here to view

To rule out the possible inhibitory effect of ethanol on collagenase activity, various ethanol concentrations (0%, 2.5%, 5%, 10%, 20%, 30%, and 40%, w/v) were tested. There was no inhibition of collagenase activity as revealed by changes in substrate concentration (fluorescence intensity) measured at 0, 15, 30, 60, 90, and 120 min [Figure 2].
Figure 2: Kinetics of the EnzChek collagenase reaction with various ethanol concentrations after incubation for 0, 15, 30, 60, 90, and 120 min. ABS: Absorbance

Click here to view

Compared with the saline + ICH rats, the striatal MMP-9 expression of the ethanol + ICH rats significantly increased at 3 and 9 h after ICH [Figure 3].
Figure 3: MMP-9 protein expression (a) representative changes in MMP-9 and β-actin at 3, 6, 9, and 24 h post-ICH insult. (b) Quantitative densitometry analysis showing relative expression of MMP-9 protein expression normalized with β-actin. Data are presented as mean ± SD *P < 0.05 versus saline + ICH (n = 6 for each group). MMP-9: Matrix metallopeptidase-9, ICH: Intracerebral hemorrhage, SD: Standard deviation

Click here to view

  Discussion Top

To study the consequences of hematoma growth after ICH insult, we adopted the collagenase injection model instead of the single blood injection model because the latter only results in a constant hematoma volume[15]. In the collagenase injection model, initial bleeding can occur as early as 10 min after induction. The volume of the hematoma progressed over 1–4 h[16],[17]. Our study showed similar hematoma growth in the saline + ICH rats: the hematoma volume stabilized at 3 h post-ICH. In contrast, the hematoma in the striata of the ethanol + ICH rats increased gradually throughout the 24 h observation period after ICH, especially after the first 3 h. To exclude a possible inhibitory effect of ethanol on collagenase, we used the EnzChek Gelatinase/Collagenase assay kit to determine the dose-dependent influence of ethanol on collagenase activity. As shown in [Figure 2], there was no direct effect of ethanol on collagenase activity.

In this study, we demonstrated pretreatment with ethanol decreased the hematoma volume at 3 h post-ICH, but aggravated hematoma formation at 9 h post-ICH. The ethanol + ICH rats had persistent hypotension until the end-point of hemodynamic monitoring, 24 h post-ICH. The ethanol did not affect the heart rate or any coagulation function tests in the rats. The concentration of ethanol (up to 40%, w/v) had no influence on the enzyme activity of the collagenase that was used to induce ICH. However, the MMP-9 in the striata of the ethanol + ICH rats significantly increased at 3 and 9 h post-ICH compared with that in the saline + ICH group.

We noted the ethanol + ICH rats exhibited less hematoma expansion than the saline + ICH rats the first 3 h after ICH [Figure 1]. Simultaneously, the ethanol-treated rats showed profound hypotension without changes in cardiac rates [Table 1]. Similar findings were mentioned by Phelan et al., who reported alcohol-intoxicated rats had significantly lower basal mean arterial pressure than controls at baseline[18]. The vasodilation induced by alcohol might contribute to the hypotensive effect after ethanol intake[19],[20]. Abdel-Rahman et al., reported ethanol could inhibit baroreflex sensitivity in conscious rats[21]. In normal rats, decreased MABP may induce tachycardia for compensation. Abdel-Rahman also reported ethanol produced a dose-related negative chronotropic effect in both Wistar rats and spontaneously hypertensive rats (SHRs), and was of longer duration in the SHR, particularly at a dose of 1 g/kg[22]. All this evidence suggests why the MABP decreased in ethanol + ICH rats without influencing their heart rates. To the best of our knowledge, the major intermediate metabolite of ethanol is acetaldehyde. Hellström and Tottmar reported only a slight decrease in mean blood pressure was seen at high blood acetaldehyde level (150–250 μM) after intravenous administration of acetaldehyde (0.5M)[23]. No effect on blood pressure was seen when the concentration of blood acetaldehyde level was lower than 50 μM in the same study. Thus, the hypotension in our experimental ICH rats was probably due to the pharmacological effect of ethanol, but not its metabolites. As a consequence, ethanol-induced hypotension might prevent hematoma volume progression in the early stage of ICH.

Larger intrastriatal hematomas emerged at 9 h, with sustainable hypotension after ICH in ethanol-treated rats. The rapid progression of hematoma enlargement might be due to coagulopathy. Further experiments revealed ethanol did not disturb coagulative functions including platelet count, PT, and aPTT. Several studies have shown ethanol intoxication did not affect fibrinolytic activity in healthy men or rats[24],[25]. Erstad et al. also demonstrated that recent ethanol exposure was not associated with significant changes in transfusion requirements or coagulation parameters in major trauma patients[26]. It is thus becoming a consensus that acute ethanol administration causes no coagulopathy or impaired hemostasis.

The ethanol + ICH rats produced more MMP-9 at 3 and 9 h post-ICH [Figure 3] than the saline + ICH rats. Interestingly, the hematomas in the ethanol + ICH animals were significantly smaller than those in the saline + ICH 3 h after ICH. Delayed hematoma expansion was demonstrated in ethanol-pretreated rats in our study. MMPs are important executors in extracellular matrix remodeling. They comprise of 8 subgroups named after their substrates: matrilysins, collagenases, stromelysins, and gelatinases[27]. MMPs can be activated by multiple pathways in the brain after ICH, for instance, hemoglobin and its derivatives[28], oxygen, or nitrogen free radicals[29], and neuroinflammatory factors[30]. On the other hand, ethanol and its metabolite, acetaldehyde, have been proven to activate MMPs through protein tyrosine kinase signaling in brain microvascular endothelial cells[14]. These activated MMPs might cause secondary brain injury, such as disruption of the BBB, brain edema, and massive neuronal death. Among these MMPs, MMP-9 is crucial for degrading basal lamina surrounding cerebral blood vessels and tight junctions of the BBB[31]. A human brain magnetic resonance imaging study revealed high BBB permeability surrounding ICH correlated to large hematomas and edema formation[32]. Hence, we proposed the ethanol-induced increment of MMPs might be responsible for the aggravated hematoma expansion after 3 h post-ICH.

  Conclusion Top

Brott et al. demonstrated that 26% patients with acute ICH had hemorrhagic expansion within the 1st h, and an additional 12% of patients had hematoma growth within 1–20 h[33]. The hematoma expansion could be predicted by a systolic BP >160 mmHg at 1.5 h after admission[34]. Recently, Rodriguez-Luna et al. also mentioned that a systolic BP >180 mmHg in the 24 h after ICH elevated the odds ratio of hematoma growth[35]. The intensive blood pressure reduction in acute cerebral hemorrhage trial proved rapid intensive lowering of blood pressure could achieve 2–4 mL absolute attenuation of hematoma growth[36]. This suggests that aggressive blood pressure control in the early stage of ICH might improve patients' clinical outcomes.

The smaller hematoma volume could be a result of sustained hypotension in the early phase (0–3 h) while increases of ethanol-induced MMP-9 might cause rapid progression of hematoma growth in the later period. The underlying mechanism between ethanol and MMP-9 activation in ICH rats still needs further investigation.

Financial support and sponsorship


Conflicts of interest

There are no conflicts of interest.

  References Top

Keep RF, Hua Y, Xi G. Intracerebral haemorrhage: Mechanisms of injury and therapeutic targets. Lancet Neurol 2012;11:720-31.  Back to cited text no. 1
Zahuranec DB, Sánchez BN, Brown DL, Wing JJ, Smith MA, Garcia NM, et al. Computed tomography findings for intracerebral hemorrhage have little incremental impact on post-stroke mortality prediction model performance. Cerebrovasc Dis 2012;34:86-92.  Back to cited text no. 2
Martini SR, Flaherty ML, Brown WM, Haverbusch M, Comeau ME, Sauerbeck LR, et al. Risk factors for intracerebral hemorrhage differ according to hemorrhage location. Neurology 2012;79:2275-82.  Back to cited text no. 3
Taylor JR, Combs-Orme T. Alcohol and strokes in young adults. Am J Psychiatry 1985;142:116-8.  Back to cited text no. 4
Duncan JW, Zhang X, Wang N, Johnson S, Harris S, Udemgba C, et al. Binge ethanol exposure increases the Kruppel-like factor 11-monoamine oxidase (MAO) pathway in rats: Examining the use of MAO inhibitors to prevent ethanol-induced brain injury. Neuropharmacology 2016;105:329-40.  Back to cited text no. 5
Maynard ME, Leasure JL. Exercise enhances hippocampal recovery following binge ethanol exposure. PLoS One 2013;8:e76644.  Back to cited text no. 6
Liew HK, Cheng HY, Huang LC, Li KW, Peng HF, Yang HI, et al. Acute alcohol intoxication aggravates brain injury caused by intracerebral hemorrhage in rats. J Stroke Cerebrovasc Dis 2016;25:15-25.  Back to cited text no. 7
Collins MA, Neafsey EJ, Mukamal KJ, Gray MO, Parks DA, Das DK, et al. Alcohol in moderation, cardioprotection, and neuroprotection: Epidemiological considerations and mechanistic studies. Alcohol Clin Exp Res 2009;33:206-19.  Back to cited text no. 8
Mukamal KJ, Chung H, Jenny NS, Kuller LH, Longstreth WT Jr., Mittleman MA, et al. Alcohol use and risk of ischemic stroke among older adults: The cardiovascular health study. Stroke 2005;36:1830-4.  Back to cited text no. 9
Wang Q, Sun AY, Simonyi A, Kalogeris TJ, Miller DK, Sun GY, et al. Ethanol preconditioning protects against ischemia/reperfusion-induced brain damage: Role of NADPH oxidase-derived ROS. Free Radic Biol Med 2007;43:1048-60.  Back to cited text no. 10
Chang JJ, Emanuel BA, Mack WJ, Tsivgoulis G, Alexandrov AV. Matrix metalloproteinase-9: Dual role and temporal profile in intracerebral hemorrhage. J Stroke Cerebrovasc Dis 2014;23:2498-505.  Back to cited text no. 11
Li N, Liu YF, Ma L, Worthmann H, Wang YL, Wang YJ, et al. Association of molecular markers with perihematomal edema and clinical outcome in intracerebral hemorrhage. Stroke 2013;44:658-63.  Back to cited text no. 12
Rubio-Araiz A, Porcu F, Pérez-Hernández M, García-Gutiérrez MS, Aracil-Fernández MA, Gutierrez-López MD, et al. Disruption of blood-brain barrier integrity in postmortem alcoholic brain: Preclinical evidence of TLR4 involvement from a binge-like drinking model. Addict Biol 2017;22:1103-16.  Back to cited text no. 13
Haorah J, Schall K, Ramirez SH, Persidsky Y. Activation of protein tyrosine kinases and matrix metalloproteinases causes blood-brain barrier injury: Novel mechanism for neurodegeneration associated with alcohol abuse. Glia 2008;56:78-88.  Back to cited text no. 14
MacLellan CL, Silasi G, Poon CC, Edmundson CL, Buist R, Peeling J, et al. Intracerebral hemorrhage models in rat: Comparing collagenase to blood infusion. J Cereb Blood Flow Metab 2008;28:516-25.  Back to cited text no. 15
Rosenberg GA, Estrada E, Kelley RO, Kornfeld M. Bacterial collagenase disrupts extracellular matrix and opens blood-brain barrier in rat. Neurosci Lett 1993;160:117-9.  Back to cited text no. 16
MacLellan CL, Davies LM, Fingas MS, Colbourne F. The influence of hypothermia on outcome after intracerebral hemorrhage in rats. Stroke 2006;37:1266-70.  Back to cited text no. 17
Phelan H, Stahls P, Hunt J, Bagby GJ, Molina PE. Impact of alcohol intoxication on hemodynamic, metabolic, and cytokine responses to hemorrhagic shock. J Trauma 2002;52:675-82.  Back to cited text no. 18
Rekik M, El-Mas MM, Mustafa JS, Abdel-Rahman AA. Role of endothelial adenosine receptor-mediated vasorelaxation in ethanol-induced hypotension in hypertensive rats. Eur J Pharmacol 2002;452:205-14.  Back to cited text no. 19
Malpas SC, Robinson BJ, Maling TJ. Mechanism of ethanol-induced vasodilation. J Appl Physiol (1985) 1990;68:731-4.  Back to cited text no. 20
Abdel-Rahman AR, Russ R, Strickland JA, Wooles WR. Acute effects of ethanol on baroreceptor reflex control of heart rate and on pressor and depressor responsiveness in rats. Can J Physiol Pharmacol 1987;65:834-41.  Back to cited text no. 21
Abdel-Rahman AA. Differential effects of ethanol on baroreceptor heart rate responses of conscious spontaneously hypertensive and normotensive rats. Alcohol Clin Exp Res 1994;18:1515-22.  Back to cited text no. 22
Hellström E, Tottmar O. Acute effects of ethanol and acetaldehyde on blood pressure and heart rate in disulfiram-treated and control rats. Pharmacol Biochem Behav 1982;17:1103-9.  Back to cited text no. 23
Hillbom M, Kaste M, Rasi V. Can ethanol intoxication affect hemocoagulation to increase the risk of brain infarction in young adults? Neurology 1983;33:381-4.  Back to cited text no. 24
Zoucas E, Bergqvist D, Göransson G, Bengmark S. Effect of acute ethanol intoxication on primary haemostasis, coagulation factors and fibrinolytic activity. Eur Surg Res 1982;14:33-44.  Back to cited text no. 25
Erstad BL, Costa CM, Daller JA, Fortune JB. Lack of hematologic effects of recent ethanol ingestion by trauma patients. Am J Ther 1999;6:299-302.  Back to cited text no. 26
Overall CM, López-Otín C. Strategies for MMP inhibition in cancer: Innovations for the post-trial era. Nat Rev Cancer 2002;2:657-72.  Back to cited text no. 27
Ding R, Feng L, He L, Chen Y, Wen P, Fu Z, et al. Peroxynitrite decomposition catalyst prevents matrix metalloproteinase-9 activation and neurovascular injury after hemoglobin injection into the caudate nucleus of rats. Neuroscience 2015;297:182-93.  Back to cited text no. 28
Fu X, Kassim SY, Parks WC, Heinecke JW. Hypochlorous acid generated by myeloperoxidase modifies adjacent tryptophan and glycine residues in the catalytic domain of matrix metalloproteinase-7 (matrilysin): An oxidative mechanism for restraining proteolytic activity during inflammation. J Biol Chem 2003;278:28403-9.  Back to cited text no. 29
Aronowski J, Zhao X. Molecular pathophysiology of cerebral hemorrhage: Secondary brain injury. Stroke 2011;42:1781-6.  Back to cited text no. 30
Rosenberg GA. Matrix metalloproteinases in neuroinflammation. Glia 2002;39:279-91.  Back to cited text no. 31
Aksoy D, Bammer R, Mlynash M, Venkatasubramanian C, Eyngorn I, Snider RW, et al. Magnetic resonance imaging profile of blood-brain barrier injury in patients with acute intracerebral hemorrhage. J Am Heart Assoc 2013;2:e000161.  Back to cited text no. 32
Brott T, Broderick J, Kothari R, Barsan W, Tomsick T, Sauerbeck L, et al. Early hemorrhage growth in patients with intracerebral hemorrhage. Stroke 1997;28:1-5.  Back to cited text no. 33
Takeda R, Ogura T, Ooigawa H, Fushihara G, Yoshikawa S, Okada D, et al. Apractical prediction model for early hematoma expansion in spontaneous deep ganglionic intracerebral hemorrhage. Clin Neurol Neurosurg 2013;115:1028-31.  Back to cited text no. 34
Rodriguez-Luna D, Piñeiro S, Rubiera M, Ribo M, Coscojuela P, Pagola J, et al. Impact of blood pressure changes and course on hematoma growth in acute intracerebral hemorrhage. Eur J Neurol 2013;20:1277-83.  Back to cited text no. 35
Delcourt C, Huang Y, Arima H, Chalmers J, Davis SM, Heeley EL, et al. Hematoma growth and outcomes in intracerebral hemorrhage: The INTERACT1 study. Neurology 2012;79:314-9.  Back to cited text no. 36


  [Figure 1], [Figure 2], [Figure 3]

  [Table 1]

This article has been cited by
1 Moderate Ethanol Pre-treatment Mitigates ICH-Induced Injury via ER Stress Modulation in Rats
Peter Bor-Chian Lin,Po-Kai Wang,Cheng-Yoong Pang,Wei-Fen Hu,Andy Po-Yi Tsai,Adrian L. Oblak,Hock-Kean Liew
Frontiers in Molecular Neuroscience. 2021; 14
[Pubmed] | [DOI]
2 Cerebral Hemorrhage and Alcohol Exposure: A Review
Jialing Peng,Hongxuan Wang,Xiaoming Rong,Lei He,L Xiangpen,Qingy Shen,Ying Peng
Alcohol and Alcoholism. 2019;
[Pubmed] | [DOI]


Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
Access Statistics
Email Alert *
Add to My List *
* Registration required (free)

  In this article
Materials and Me...
Article Figures
Article Tables

 Article Access Statistics
    PDF Downloaded285    
    Comments [Add]    
    Cited by others 2    

Recommend this journal